Operator assisted acquisition and analysis
Operator unassisted analysis and acquisition (for individuals who have attended the instructional class)
Off-line data analysis
Calcium flux analysis
Fluorescence activated cell sorting
Sample staining and analysis
Operator unassisted use of the RoboSep cell separator
Reagents and disks are available for purchase. Instructional sessions on the operation of the analytical flow cytometers and the use of the MACS are also offered.
There is no charge for consultation and assistance with experimental design. Also, new methods that will be generally useful for the UCLA flow cytometry community are developed without charge.
UCLA and
Affiliate Rates
Service/Unit
|
JCCC Members |
CFAR Members |
UCLA non-JCCC,
non CFAR |
| Sorting/hr | $82 |
$85 |
$96 |
| Acquisition (Tech)/hr | $82 |
$84 |
$88 |
| Acquisition (Indep)/hr | $57 |
$60 |
$68 |
| RoboSep/ 45 min | $30 |
$28.50 |
$37.50 |
| Instructional Sessions | |||
| Analytic Cytometry Instructional Course (1day) | $272 |
$280 |
$336 |
| DNA Class (4hrs) | $136 |
$140 |
$168 |
| RoboSep Instructional Session (1hr) | FREE |
FREE |
FREE |
| Indep Data Analysis/hr | $34 |
$36 |
$41 |
| Staining and Analysis (2 tube minimum) | see below |
see below |
see below |
| Single Color |
$20 |
$20 |
$20 |
Dual Color |
$40 |
$40 |
$40 |
Triple Color |
$60 |
$60 |
$60 |
| Monoclonal Antibodies (Stains 5x105 Cells) | $4.00/Aliquot |
$4.00/Aliquot |
$4.00/Aliquot |
| Disks | |||
1 GB USB |
$20 |
$20 |
$20 |
To defray the high operating costs to the Core Facility, it receives considerable financial support through NIH grants to the Jonsson Comprehensive Cancer Center, the JCCC, and the Center for the AIDS Research, and is also subsidized directly by the JCCC, the UCLA AIDS Institute and the David Geffen School of Medicine at UCLA.
YOU CAN HELP! To continue to receive these subsidies, we must be able to prove that the Core provides a useful service and enhances research at UCLA. The best way to show this is for investigators to acknowledge the Core Facility and its funding sources in every publication that contains data acquired in the Flow Cytometry Core Facility. If you then provide us with a title page, or a reprint, we can report those publications as progress in our grant continuations and renewals.
A suggested acknowledgement is:
Flow cytometry was performed in the UCLA Jonsson Comprehensive Cancer Center (JCCC) and Center for AIDS Research Flow Cytometry Core Facility that is supported by National Institutes of Health awards CA-16042 and AI-28697, and by the JCCC, the UCLA AIDS Institute, the David Geffen School of Medicine at UCLA, and the UCLA Chancellor's Office.
Please help! Otherwise, UCLA investigators will have to pay the unsubsidized recharge rates for non-UCLA affiliates (see below).
Users who are not affiliated with UCLA
are required to pay the unsubsidized rates as listed below.
Non-UCLA Rates
Service/Unit |
Price |
| Sorting/hr | $163 |
| Acquisition (Tech)/hr | $160 |
| Acquisition (Indep)/hr | $115 |
| RoboSep/ 45 min | $58.50 |
| Instructional Sessions | |
| Analytic Cytometry Instructional Course (1day) | $568 |
| DNA Class (4hrs) | $284 |
| RoboSep Instructional Session (1hr) | FREE |
| Indep Data Analysis/hr | $69 |
| Staining and Analysis (2 tube minimum) | |
Single Color |
$20/tube |
Dual Color |
$40/tube |
Triple Color |
$60/tube |
| Monoclonal Antibodies (Stains 5x105 Cells) | $4/Aliquot |
| Disks | |
1 GB USB |
$20 |
Core facility billing of on-campus laboratories is conducted based on monthly internal recharge identification number basis. Off-campus laboratories will be invoiced and can pay by check.