UCLA FlowCytometry Core Facility

Courses Overview

The UCLA Core facility offers two flow cytometry courses. One class teaches basic flow cytometry principles in addition to offering hands-on training on the operation of Becton Dickinson analytic flow cytometers. The other course teaches principles of nucleic acid analysis by flow cytometry as well as hands-on instrument set-up and proper use of the specialized DNA analysis software. In addition, the Core facility provides individual training in the use of the RoboSep magnetic cell sorter which is mandatory for all new users of the RoboSep. To arrange for this training, please contact Ingrid Schmid.

Basic Flow Cytometry Course

Who should take this course?

What benefits does it provide?

Theoretical Tutorial Outline

  1. Components of flow cytometers (fluidics, lasers, filters, detectors)
  2. Fluorescence absorption and emission
  3. Spectral overlap
  4. Fluorescence compensation
  5. Fluorescence intensity measurements
  6. Data analysis

Hands-On Tutorial Outline

  1. A brief overview of Core Laboratory Policies
  2. Biosafety considerations
  3. Introduction to instrument set-up
  4. Introduction to CellQuest software
  5. Introduction to gating principles
  6. Set up of multi-color flow cytometry
  7. Electronic compensation adjustments
  8. Data analysis techniques
  9. Troubleshooting tips

Course Fees

Compared to the cytometer manufacturer’s courses, the UCLA Core flow cytometry course is an excellent value for UCLA faculty, staff, and students. Please, refer to the services section for the class fee associated with your membership status. This fee includes pre-course reading material and a course booklet. If you have to cancel your appointment, please call 24 hours in advance. If you fail to do so, we reserve the right to charge the entire course fee.

Registration and Times

To register for this course please contact Ingrid Schmid. You will be contacted with information about course availability and date. The course is held on an as-needed basis, usually once every month, most often on Mondays and Tuesdays. These one-day classes, held in the Core facility, begin at 9:00 a.m. and end at approximately 4:45 p.m. There is a one hour lunch break and two short breaks; one in the morning and one in the afternoon. A minimum of four confirmed students is needed to arrange a suitable class date. The maximum class size is eight students.

Nucleic Acid Flow Cytometry Course

Who should take this course?

Theoretical Tutorial Outline

  1. Brief Introduction to Flow Cytometry
  2. Fundamentals of Nucleic Acid Analysis
    • Cell Cycle Phases
    • The DNA Histogram
  3. Stem Cell "side population"
  4. Cell Cycle Kinetics
    • Pulse processing
    • Nucleic Acid Stains and their Properties
  5. DNA/RNA Measurements
  6. Proliferation
    • Bromodeoxyuridine
    • EDU Click-iT Technology
    • Pulse-Chase Experiment, Cell Cycle Arrest
    • Dye Dilution Assay
  7. Ploidy
  8. Chromosome Analysis
  9. Measurement of Apoptosis and Dead Cell Discrimination
  10. Combination of Nucleic Acid with Cell Surface Antigen Analysis
  11. Combination of Nucleic Acid with Intracellular Protein Analysis

Hands-On Tutorial Outline

  1. Sample Acquisition for DNA content analysis (Optimizing Settings, Recognizing Aggregates/Debris)
  2. ModFit™ Analysis (Used for DNA Histogram Analysis)
  3. Analysis of Nucleic Acid Stained Samples with CellQuest™
    • Dead Cell Discrimination
    • Apoptosis

Course Fees

Compared to commercial courses, the UCLA Nucleic Acid and Proliferation flow cytometry course is an excellent value for UCLA faculty, staff and students. Please, refer to the services section for the DNA class fee associated with your membership status. Fees include pre-course reading material and a course booklet. If you have to cancel your appointment, please call 24 hours in advance. If you fail to do so, we reserve the right to charge the entire course fee.

Registration and Times

To register for the nucleic acid course please call Ingrid Schmid. You will be contacted with information about course availability and date. The course is held on an as-needed basis. A minimum of four confirmed students is needed to arrange a suitable class date. The maximum class size is eight students.